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KMID : 0379520160320030251
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한국독성학회지
2016 Volume.32 No. 3 p.251 ~ p.258
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Tumorigenicity Evaluation of Umbilical Cord Blood-derived Mesenchymal Stem Cells
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Park Sang-Jin
Kim Hyun-Jung
Kim Woo-Jin
Kim Ok-Sun
Lee Sun-Yeong
Han Su-Yeon
Jeong Eun-Ju
Park Hyun-Shin
Kim Hea-Won
Moon Kyoung-Sik
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Abstract
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Mesenchymal stem cells (MSCs) have been identified in multiple types of tissue and exhibit characteristic self-renewal and multi-lineage differentiation abilities. However, the possibility of oncogenic transformation after transplantation is concerning. In this study, we investigated the tumorigenic potential of umbilical cord blood-derived MSCs (hUCB-MSCs) relative to MRC-5 and HeLa cells (negative and positive controls, respectively) both in vitro and in vivo. To evaluate tumorigenicity in vitro, anchorage-independent growth was assessed using the soft agar colony formation assay. hUCB-MSCs and MRC-5 cells formed few colonies, while HeLa cells formed a greater number of larger colonies, indicating that hUCB-MSCs and MRC-5 cells do not have anchorage-independent proliferation potential. To detect tumorigenicity in vivo, hUCB-MSCs were implanted as a single subcutaneous injection into BALB/c-nu mice. No tumor formation was observed in mice transplanted with hUCB-MSCs or MRC-5 cells based on macroand microscopic examinations; however, all mice transplanted with HeLa cells developed tumors that stained positive for a human gene according to immunohistochemical analysis. In conclusion, hUCB-MSCs do not exhibit tumorigenic potential based on in vitro and in vivo assays under our experimental conditions, providing further evidence of their safety for clinical applications.
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KEYWORD
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Tumorigenicity, Mesenchymal stem cells, Immunohistochemistry, Soft agar assay
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